apaf1 (Proteintech)
Structured Review
Apaf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+igf2bp3+antibody/IGF2BP3+Antibody/pm41787385-145-38-41
Average 96 stars, based on 208 article reviews
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Incubation:Article Title: VIRMA/IGF2BP3-mediated ANLN upregulation promotes intrahepatic cholangiocarcinoma growth by forming a positive feedback loop with RhoA/YAP1/TEAD1 signaling pathway. Article Snippet: .. Following this, the cell lysates were precoated and incubated with 5mg of Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability. Article Snippet: 2.9 RIP-qPCR assay RNA immunoprecipitation (RIP) was performed using the BeyoRIPTM RIP assay kit (P1801S, Beyotime) to investigate IGF2BP3ID1 mRNA interactions. .. Following lysis of infected MG-63 cells, supernatants were incubated overnight at 4°C with pre-blocked Protein A/G agarose beads conjugated to Article Title: Interaction between IGF2BP3 and RP11-480I12.5 facilitates proliferation and suppresses apoptosis in non-small cell lung cancer. Article Snippet: .. Subsequently, the slides underwent permeabilization with a 0.5% Triton X-100 solution (X100, Amresco, OH, USA) for 5 min, and were then blocked with 10% goat serum in 0.1% Triton X-100 PBS for a period of 1 h. The primary Article Title: VIRMA/IGF2BP3-mediated ANLN upregulation promotes intrahepatic cholangiocarcinoma growth by forming a positive feedback loop with RhoA/YAP1/TEAD1 signaling pathway Article Snippet: .. Following this, the cell lysates were pre-coated and incubated with 5 mg of Article Title: Interaction between IGF2BP3 and RP11-480I12.5 facilitates proliferation and suppresses apoptosis in non-small cell lung cancer Article Snippet: Subsequently, the protein samples were separated by 12% SDS–PAGE, and transferred onto 0.45 μm PVDF membranes (B1000B, Invitrogen, CA, USA) [ ]. .. After being blocked with 5% skim milk, the membranes were incubated with the primary Article Title: Interaction between IGF2BP3 and RP11-480I12.5 facilitates proliferation and suppresses apoptosis in non-small cell lung cancer Article Snippet: .. Subsequently, the slides underwent permeabilization with a 0.5% Triton X-100 solution (X100, Amresco, OH, USA) for 5 min, and were then blocked with 10% goat serum in 0.1% Triton X-100 PBS for a period of 1 h. The primary Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability Article Snippet: RNA immunoprecipitation (RIP) was performed using the BeyoRIPTM RIP assay kit (P1801S, Beyotime) to investigate IGF2BP3-ID1 mRNA interactions. .. Following lysis of infected MG-63 cells, supernatants were incubated overnight at 4 °C with pre-blocked Protein A/G agarose beads conjugated to Article Title: Interaction between IGF2BP3 and RP11-480I12.5 facilitates proliferation and suppresses apoptosis in non-small cell lung cancer. Article Snippet: Subsequently, the protein samples were separated by 12% SDS–PAGE, and transferred onto 0.45 μm PVDF membranes (B1000B, Invitrogen, CA, USA) [26]. .. After being blocked with 5% skim milk, the membranes were incubated with the primary Lysis:Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability. Article Snippet: 2.9 RIP-qPCR assay RNA immunoprecipitation (RIP) was performed using the BeyoRIPTM RIP assay kit (P1801S, Beyotime) to investigate IGF2BP3ID1 mRNA interactions. .. Following lysis of infected MG-63 cells, supernatants were incubated overnight at 4°C with pre-blocked Protein A/G agarose beads conjugated to Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability Article Snippet: RNA immunoprecipitation (RIP) was performed using the BeyoRIPTM RIP assay kit (P1801S, Beyotime) to investigate IGF2BP3-ID1 mRNA interactions. .. Following lysis of infected MG-63 cells, supernatants were incubated overnight at 4 °C with pre-blocked Protein A/G agarose beads conjugated to Infection:Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability. Article Snippet: 2.9 RIP-qPCR assay RNA immunoprecipitation (RIP) was performed using the BeyoRIPTM RIP assay kit (P1801S, Beyotime) to investigate IGF2BP3ID1 mRNA interactions. .. Following lysis of infected MG-63 cells, supernatants were incubated overnight at 4°C with pre-blocked Protein A/G agarose beads conjugated to Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability Article Snippet: RNA immunoprecipitation (RIP) was performed using the BeyoRIPTM RIP assay kit (P1801S, Beyotime) to investigate IGF2BP3-ID1 mRNA interactions. .. Following lysis of infected MG-63 cells, supernatants were incubated overnight at 4 °C with pre-blocked Protein A/G agarose beads conjugated to Control:Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability Article Snippet: RNA immunoprecipitation (RIP) was performed using the BeyoRIPTM RIP assay kit (P1801S, Beyotime) to investigate IGF2BP3-ID1 mRNA interactions. .. Following lysis of infected MG-63 cells, supernatants were incubated overnight at 4 °C with pre-blocked Protein A/G agarose beads conjugated to |
